Reproduction Abstracts

The aim of this study was to analyze the effects of refrigeration and cryopreservation on dog sperm special on sperm motility and mitochondrial membrane potential (MMP). A total of 15 ejaculates, first and second fractions, were collected from five dogs. Semen was diluted (80×106 sptz/ml) on Tris-egg-yolk medium with 8% of glycerol (one step), filled into 0.5 ml French straw and refrigerated at 5 °C for 1 h. After straws were suspended 6 cm above liquid ni...